Research Article

Amine Analysis Using AlexaFluor 488 Succinimidyl Ester and Capillary Electrophoresis with Laser-Induced Fluorescence

Figure 1

AlexaFluor 488 (AF488) succinimidyl ester and its reaction with primary amines. (a) The chemical structure of the reaction of AF488 succinimidyl ester with primary amines. N-hydroxysuccinimide acts as a leaving group to promote the formation of an amide bond to link AF488 to the primary amine group.
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