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Journal of Analytical Methods in Chemistry
Volume 2015, Article ID 404925, 6 pages
http://dx.doi.org/10.1155/2015/404925
Research Article

Paclobutrazol Residue Determination in Potato and Soil Using Low Temperature Partition Extraction and Ultrahigh Performance Liquid Chromatography Tandem Mass Spectrometry

1Supervision & Testing Center for Farm Product Quality, Ministry of Agriculture, Kunming, China
2Laboratory of Quality & Safety Risk Assessment for Agro-Product, Ministry of Agriculture, Kunming, China
3Institute of Quality Standard and Testing Technology, Yunnan Academy of Agriculture Science, Kunming 650223, China
4Institute of Agriculture Environment and Source, Yunnan Academy of Agriculture Science, Kunming 650223, China
5Kunming Medical University, Kunming, China

Received 1 May 2015; Revised 3 July 2015; Accepted 8 July 2015

Academic Editor: Juan F. Garcia-Reyes

Copyright © 2015 Hongcheng Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A simple, accurate, and highly sensitive analytical method was developed for determining the paclobutrazol residue in potato and soil, the dynamics dissipation in soil. Extraction was carried out by low temperature partitioning and analyzed by ultrahigh performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). For a favor extraction yield, the parameters such as temperature and solvent were optimized. The result showed that sample would be easily frozen and separated using acetonitrile under −20°C for 10 min. The limit of detection (LOD) was 0.5 μg/kg, and the limit of quantification (LOQ) was 2 and 5 μg/kg for potato and soil, respectively. The influence of paclobutrazol residue in potato was evaluated. The possible contamination of paclobutrazol from surface can be rinsed by distilled water or peeled off, but the paclobutrazol in potato harvest comes mainly from absorption and transport, which could not be removed by peeling. The half-life of paclobutrazol in soil was 20.64 days, and the residue was below 0.22 mg/kg on 50th day after spraying. According to the risk assessment with Need Maximum Daily Intake (NEDI) and Acceptable Daily Intake (ADI), a Maximum Residue Limit (MRL) of paclobutrazol in potato was recommended as 1.0 mg/kg.