Research Article

Analysis of Reaction between -Lipoic Acid and 2-Chloro-1-methylquinolinium Tetrafluoroborate Used as a Precolumn Derivatization Technique in Chromatographic Determination of -Lipoic Acid

Table 2

Validation parameters of elaborated methods.

Parameter Direct spectrophotometric methodSpectrophotometric method based on derivatization reaction HPLC method based on DHLA-CMQT product as precolumn derivatization reaction

Linearity range/μmol L−150–7501.0–10 2.5–50
Equation of calibration curve/-concentration in mol L−1 = 1.03 + 1.0−3 = 1.04 + 0.0−3 = 4.07
Correlation coefficient0.9990.9990.997
LOD/μmol L−11.1200.258 0.214
LOQ/μmol L−1370.784 0.647
RSD/%0.53.02.06
SD0.0180−30.014