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Journal of Analytical Methods in Chemistry
Volume 2015, Article ID 589654, 11 pages
Research Article

A Useful Strategy to Evaluate the Quality Consistency of Traditional Chinese Medicines Based on Liquid Chromatography and Chemometrics

1School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China
2Center for Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, North Carolina Research Campus, 500 Laureate Way, Kannapolis, NC 28081, USA

Received 21 August 2015; Accepted 11 October 2015

Academic Editor: Sibel A. Ozkan

Copyright © 2015 Pei Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Online Supporting Information 1: Development of the four typical fingerprints included single wavelength fingerprint, 3D fingerprint, Maximum absorption wavelength fingerprint, and multi-wavelength fingerprint.

Supplemental Figure 1. Similarities between the fingerprint of each sample and the standard fingerprint.

Supplemental Figure 2. Fingerprint of XKS and the five individual herbals. Fingerprint of XKS was the mean fingerprint derived from the fingerprints of 71-batch samples by average method generated using software named “the similarity evaluation system for TCM”. The common peaks in XKS fingerprint were assigned to each crude herb. For the 35 common peaks, 13 of them were from Pueraria lobatae radix (Chinese name Gegen, GG), 7 of them were from Salviae Miltiorrhizae Radix et Rhizoma (Chinese name Danshen, DS), 13 of them were form Crataegi Fructus (Chinese name Shanzha, SZ), and 2 of them were from Aucklandiae Radix (Chinese name Muxiang, MX). We cannot found any peaks belong to Radix Notoginseng in the fingerprint of XKS tablet, since for Notoginseng Radix et Rhizoma (Chinese name Sanqi, SQ), the major bioactive constituents are saponins. For this type of constituents, the maximum UV absorbance wavelength is 203 nm, thus, we built another quantification method for the quality control of the saponins in XKS tablet (data not shown). All the common peaks were identified by LC-MS/MS. The peaks were assigned to each crude herb based on both the MS information and the retention times (data not shown).

  1. Supplementary Material