Quality Assessment of Ojeok-San, a Traditional Herbal Formula, Using High-Performance Liquid Chromatography Combined with Chemometric Analysis

Table 3

Intra- and interday precision of the marker compounds of OJS.

Compound	Spiked concentration (g/mL)	Intraday (n = 3)			Interday ()
		Detected concentration (g/mL)	RSD^a (%)	Accuracy (%)	Detected concentration (g/mL)	RSD (%)	Accuracy (%)
Gallic acid	1.00	1.00	1.68	100.41	1.00	1.68	100.41
	2.00	2.00	0.37	100.18	2.00	0.48	100.12
Protocatechuic acid	1.00	1.02	0.89	101.59	1.00	1.79	100.11
	2.00	2.00	0.45	99.97	2.01	0.43	100.31
Chlorogenic acid	2.00	1.96	0.27	98.04	1.96	0.38	97.98
	4.00	4.02	0.06	100.49	4.02	0.09	100.51
Albiflorin	2.00	1.99	1.09	99.56	1.97	2.57	98.61
	4.00	4.00	0.27	100.11	4.02	0.45	100.52
Paeoniflorin	10.00	10.22	1.36	102.20	10.22	1.37	102.20
	20.00	19.89	0.35	99.45	19.89	0.35	99.45
Ferulic acid	2.00	2.01	0.56	100.53	1.99	1.10	99.62
	4.00	4.00	0.26	100.09	4.00	0.27	100.10
Liquiritin	5.00	5.22	1.13	104.46	5.24	1.50	104.80
	10.00	9.89	0.30	98.88	9.88	0.40	98.80
Benzoic acid	3.00	2.88	0.67	95.97	2.87	1.22	95.67
	6.00	6.06	0.16	101.01	6.06	0.29	101.08
Nodakenin	5.00	5.01	0.42	100.12	5.03	0.28	100.51
	10.00	10.00	0.11	99.97	9.99	0.16	99.93
Hesperidin	20.00	20.59	0.39	102.97	20.60	0.41	102.98
	40.00	39.70	0.10	99.26	39.70	0.11	99.25
Naringin	20.00	20.83	0.30	104.15	20.83	0.31	104.15
	40.00	39.58	0.08	98.96	39.58	0.08	98.96
Neohesperidin	15.00	14.11	0.15	94.07	14.10	0.23	94.02
	30.00	30.44	0.03	101.48	30.46	0.02	101.53
Ononin	1.00	0.98	0.26	97.60	0.98	0.24	97.60
	2.00	2.01	0.06	100.60	2.01	0.06	100.60
Oxypeucedanin hydrate	1.00	0.98	1.99	97.91	0.97	2.65	97.35
	2.00	2.01	0.49	100.52	2.01	0.64	100.66
Cinnamic acid	1.00	0.99	0.09	99.26	0.99	0.50	99.02
	2.00	2.00	0.02	100.19	2.00	0.12	100.24
Byakangelicin	1.00	0.99	1.53	99.25	0.99	1.87	99.01
	2.00	2.01	0.22	100.49	2.01	0.36	100.59
Cinnamaldehyde	1.05	1.02	0.63	97.43	1.03	0.93	97.64
	2.10	2.11	0.31	100.27	2.11	0.34	100.29
Benzoylpaeoniflorin	1.00	1.01	1.53	100.87	1.00	1.29	99.81
	2.00	2.00	0.32	100.05	2.00	0.45	99.97
Glycyrrhizin	3.00	2.93	0.38	97.53	2.92	0.68	97.35
	6.00	6.04	0.09	100.62	6.04	0.16	100.66

RSD (%) = (SD/mean) 100.