Regularities of Anthocyanins Retention in RP HPLC for “Water–Acetonitrile–Phosphoric Acid” Mobile Phases

<div>Flavylium form of 3-glycosides of six common anthocyanidins. <svg height="12.3101pt" id="M1" style="vertical-align:-3.427739pt" version="1.1" viewbox="-0.0498162 -8.88236 16.7673 12.3101" width="16.7673pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0135,0,0,-0.0135,0,0)"><path d="M631 18C609 24 585 35 559 65C534 91 514 117 478 169C448 214 406 281 389 313C462 346 516 399 516 485C516 545 490 590 449 616C412 641 363 650 290 650H42V622C120 615 128 612 128 527V125C128 40 120 34 38 28V0H300V28C221 34 212 40 212 125V284H244C295 284 312 272 329 244C359 195 395 133 430 84C475 19 516 -3 592 -7C603 -8 615 -8 627 -8L631 18ZM212 316V563C212 591 215 602 223 607C231 613 248 617 277 617C352 617 423 577 423 469C423 415 407 375 368 345C343 324 310 316 260 316H212Z" id="g190-83"></path><glyph.data ascent="989" descent="-360" horiz-adv-x="621" vert-adv-y="621"></glyph.data></g><g transform="matrix(.0095,0,0,-0.0095,8.445,3.264)"><path d="M290 377C321 398 342 415 358 430C378 450 389 473 389 502C389 578 329 635 238 635H237C184 635 137 610 109 578L64 515L88 493C112 529 154 573 208 573S303 542 303 482C303 409 233 370 141 341L149 308C165 313 190 319 215 319C272 319 341 283 341 193C342 98 292 43 222 43C163 43 122 72 96 94C88 101 79 100 70 94C61 87 47 73 46 60C44 47 48 37 62 23C76 10 118 -12 165 -12C238 -12 430 62 430 223C430 297 379 359 290 375V377Z" id="g50-52"></path><glyph.data ascent="3443" descent="-2856" horiz-adv-x="487" vert-adv-y="487"></glyph.data></g><g transform="matrix(.0068,0,0,-0.0068,13.064,-.068)"><path d="M323 549L316 585C302 600 218 636 190 624L84 81L142 54L323 549Z" id="g176-31"></path><glyph.data ascent="3439" descent="-2858" horiz-adv-x="381" vert-adv-y="381"></glyph.data></g></svg> and <svg height="12.3101pt" id="M2" style="vertical-align:-3.427739pt" version="1.1" viewbox="-0.0498162 -8.88236 16.7673 12.3101" width="16.7673pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0135,0,0,-0.0135,0,0)"><path d="M631 18C609 24 585 35 559 65C534 91 514 117 478 169C448 214 406 281 389 313C462 346 516 399 516 485C516 545 490 590 449 616C412 641 363 650 290 650H42V622C120 615 128 612 128 527V125C128 40 120 34 38 28V0H300V28C221 34 212 40 212 125V284H244C295 284 312 272 329 244C359 195 395 133 430 84C475 19 516 -3 592 -7C603 -8 615 -8 627 -8L631 18ZM212 316V563C212 591 215 602 223 607C231 613 248 617 277 617C352 617 423 577 423 469C423 415 407 375 368 345C343 324 310 316 260 316H212Z" id="g190-83"></path><glyph.data ascent="989" descent="-360" horiz-adv-x="621" vert-adv-y="621"></glyph.data></g><g transform="matrix(.0095,0,0,-0.0095,8.445,3.264)"><path d="M158 548H390L417 615L410 623H122L83 318C105 326 143 337 185 337C296 337 350 275 350 188C350 116 308 42 225 42C164 42 122 74 100 93C90 101 82 99 72 92C60 82 51 68 50 59C48 46 52 38 66 24C82 9 125 -12 172 -12C225 -11 292 15 346 59C408 108 437 166 437 226C437 309 371 397 242 397C214 397 170 382 133 369L158 548Z" id="g50-54"></path><glyph.data ascent="3443" descent="-2856" horiz-adv-x="487" vert-adv-y="487"></glyph.data></g><g transform="matrix(.0068,0,0,-0.0068,13.064,-.068)"><path d="M323 549L316 585C302 600 218 636 190 624L84 81L142 54L323 549Z" id="g176-31"></path><glyph.data ascent="3439" descent="-2858" horiz-adv-x="381" vert-adv-y="381"></glyph.data></g></svg> are H and H for pelargonidin (Pg); OH and H for cyanidin (Cy); OH and OH for delphinidin (Dp); OCH<sub>3</sub> and H for peonidin (Pn); OCH<sub>3</sub> and OH for petunidin (Pt); OCH<sub>3</sub> and OCH<sub>3</sub> for malvidin (Mv).</div>

Journal of Analytical Methods in Chemistry

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Figure 1

Figure 1: Regularities of Anthocyanins Retention in RP HPLC for “Water–Acetonitrile–Phosphoric Acid” Mobile Phases 