Development of a Gradient HPLC Method for the Simultaneous Determination of Sotalol and Sorbate in Oral Liquid Preparations Using Solid Core Stationary Phase

Figure 2

Chromatograms of the standard solution (SOT 0.2 mg mL⁻¹, SORB 0.04 mg mL⁻¹, and EP 0.08 mg mL⁻¹), sample solution (1.000 mL of pharmaceutical preparation and 1.000 mL of stock solution of internal standard EP diluted to 25.00 mL), and blank solution (1.000 mL of placebo diluted to 25.00 mL); injection volume 5 μL; mobile phase flow 1.3 mL min⁻¹; linear gradient (ACN: 10% to 60% in 4 minutes); UV/Vis detector wavelength 237 nm; column oven 25°C.