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Journal of Analytical Methods in Chemistry
Volume 2015, Article ID 819067, 8 pages
Research Article

Optimization of Gentisides Extraction from Gentiana rigescens Franch. ex Hemsl. by Response Surface Methodology

1Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China
2College of Traditional Chinese Medicine, Yunnan College of Traditional Chinese Medicine, Kunming 650500, China
3Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming 650200, China

Received 26 September 2015; Revised 12 November 2015; Accepted 6 December 2015

Academic Editor: Ricardo Jorgensen Cassella

Copyright © 2015 Bowen Chu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Gentisides are a class of chemical compounds which is considered as potential therapeutic substance for treatment of neurodegenerative disorders. The heat reflux extraction conditions were optimized for seven kinds of gentisides from the root and rhizome of Gentiana rigescens Franch. ex Hemsl. by employing response surface method. Based on univariate test, a Box-Behnken design (BBD) was applied to the survey of relationships between response value (gentisides yield) and independent variables which were chosen from various extraction processes, including extraction temperature, extraction time, and solvent-material ratio. The optimized conditions for this extraction are as follows: extraction time of 3.40 h, extraction temperature of 74.33°C, and ratio of solvent to raw material of 10.21 : 1 mL/g. Verification assay revealed that the predicted value (99.24%) of extraction parameters from this model was mainly conformed to the experimentally observed values ().