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Journal of Analytical Methods in Chemistry
Volume 2015, Article ID 819067, 8 pages
Research Article

Optimization of Gentisides Extraction from Gentiana rigescens Franch. ex Hemsl. by Response Surface Methodology

1Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China
2College of Traditional Chinese Medicine, Yunnan College of Traditional Chinese Medicine, Kunming 650500, China
3Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming 650200, China

Received 26 September 2015; Revised 12 November 2015; Accepted 6 December 2015

Academic Editor: Ricardo Jorgensen Cassella

Copyright © 2015 Bowen Chu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Material 1: Gentisides were series of new neuritogenic activity compounds isolated from G. rigescens, and their bioactivity was related to only alkyl chain length, not structural diversity of end of the alkyl chain. Chemical structural formula of seven kinds of gentiside, namely gentiside A, gentiside B, gentiside G, gentiside H, gentiside I, gentiside J, and gentiside K, had been drawn as following.

Supplementary Material 2: Based on the univariate test, extraction temperature, extraction time and ratio of liquid to material were selected as three-factors in BBD experiment. And three level points in investigated range were respectively coded as -1, 0 and +1 for the sake of experimental design.

Supplementary Material 3: According to calculating by seven various concentrations, seven created regression equations performed a good linear relationship in given linear ranges and the correlation coefficients (R2) were ≥0.99 for all of the compounds over corresponding linearity range.

Supplementary Material 4: To ensure our experimental equipment and data to be reliable, HPLC instrument received a validation including precision, stability, repeatability and recovery in this work. In order to assess the intra-day and inter-day precision of the method, standard solution was analyzed in a day (0, 2, 4, 8, 16 and 24 h) and three consecutive days (0, 24, 36, 48, 60 and 72 h), respectively. To test the stability of samples, unitary extract solution was analyzed by HPLC within a day (0, 2, 4, 8, 16 and 24 h). To confirm the repeatability, six repeated samples were once test. The RSD (%) of each item less than 3 meant the described method had an accepted degree of precision.

  1. Supplementary Material