Development and Validation of an HPLC Method for Simultaneous Quantification of Clopidogrel Bisulfate, Its Carboxylic Acid Metabolite, and Atorvastatin in Human Plasma: Application to a Pharmacokinetic Study

Table 1

Gradient program for simultaneous determination of clopidogrel, its carboxylic acid metabolite, and atorvastatin.

Time	Solvent A	Solvent B	Solvent C
(min)	(Buffer solution)	(ACN)	(CH3OH)
0.01	90	10	0
2.00	90	10	0
3.00	60	40	0
4.00	30	50	20
8.00	30	50	20
10.00	30	40	30
18.00	30	50	20
19.00	90	10	0
20.00	90	10	0