Development and Validation of an HPLC Method for Simultaneous Quantification of Clopidogrel Bisulfate, Its Carboxylic Acid Metabolite, and Atorvastatin in Human Plasma: Application to a Pharmacokinetic Study

Table 2

Intraday and interday precision and accuracy of the method.

Analyte	Period of analysis	Nominal concentration μg·mL^−1	Mean concentration found μg·mL^−1	Recovery %	RSD %
CLP	Intraday	0.25	0.24 ± 0.01	96	4.17	3
		0.50	0.49 ± 0.02	98	4.08	3
		1	0.97 ± 0.02	97	2.06	3
		2	1.97 ± 0.04	98	2.03	3
	Interday	0.25	0.24 ± 0.02	96	8.33	3
		0.50	0.48 ± 0.01	96	2.08	3
		1	0.96 ± 0.02	96	2.08	3
		2	1.94 ± 0.05	97	2.57	3
CCA	Intraday	0.5	0.47 ± 0.02	94	4.26	3
		1	0.95 ± 0.01	95	1.05	3
		2	1.96 ± 0.02	98	1.02	3
		4	3.87 ± 0.08	97	2.07	3
	Interday	0.50	0.47 ± 0.01	94	2.12	3
		1	0.96 ± 0.01	96	1.04	3
		2	1.95 ± 0.03	98	1.54	3
		4	3.88 ± 0.08	97	2.06	3
ATV	Intraday	0.20	0.18 ± 0.01	90	5.55	3
		0.3	0.28 ± 0.01	93	3.57	3
		1	0.97 ± 0.01	97	1.03	3
		2.5	2.44 ± 0.03	98	1.23	3
	Interday	0.20	0.18 ± 0.01	90	5.56	3
		0.30	0.29 ± 0.02	97	6.90	3
		1	0.98 ± 0.05	98	5.10	3
		2.5	2.47 ± 0.01	99	0.40	3

CLP: clopidogrel bisulfate; CCA: clopidogrel carboxylic acid metabolite; ATV: atorvastatin.