Development and Validation of an UHPLC-QqQ-MS Technique for Simultaneous Determination of Ten Bioactive Components in Fangji Huangqi Tang

Table 2

Calibration curves, LOD, and LOQ of the 10 major compounds.

Compounds	Calibration curve		Linear range (ng/mL)	LOQ (ng/mL)	LOD (ng/mL)	MS/MS ()	Detected ion
Tetrandrine		0.9996	1.63–104.16	0.36	0.11	623.2/381.3	[M + H]^+
Fangchinoline		0.9991	1.84–117.54	0.18	0.05	609.3/367.1	[M + H]^+
Atractylenolide I		0.9998	0.37–23.55	0.09	0.03	233.1/187.2	[M + H]^+
Atractylenolide III		0.9993	0.56–35.63	0.43	0.13	249.1/231.0	[M + H]^+
Calycosin-7-O-β-D-glucoside		0.9999	9.94–635.92	0.02	0.01	447.2/285.1	[M + H]^+
Glycyrrhizin		0.9991	31.40–2009.56	1.89	0.57	821.3/351.2	[M − H]^−
Liquiritin		0.9996	123.75–7920.00	0.26	0.08	417.1/255.1	[M − H]^−
Isoliquiritin		0.9991	30.38–1944.03	0.18	0.05	417.1/255.1	[M − H]^−
Liquiritigenin		0.9995	2.12–135.55	0.61	0.18	256.2/135.0	[M − H]^−
Isoliquiritigenin		0.9998	0.61–39.01	0.48	0.14	256.2/135.0	[M − H]^−