Table 2: Calibration curves, LOD, and LOQ of the 10 major compounds.

CompoundsCalibration curveLinear range (ng/mL)LOQ (ng/mL)LOD (ng/mL)MS/MS ()Detected ion

Tetrandrine0.99961.63–104.160.360.11623.2/381.3[M + H]+
Fangchinoline0.99911.84–117.540.180.05609.3/367.1[M + H]+
Atractylenolide I0.99980.37–23.550.090.03233.1/187.2[M + H]+
Atractylenolide III0.99930.56–35.630.430.13249.1/231.0[M + H]+
Calycosin-7-O-β-D-glucoside0.99999.94–635.920.020.01447.2/285.1[M + H]+
Glycyrrhizin0.999131.40–2009.561.890.57821.3/351.2[M − H]
Liquiritin0.9996123.75–7920.000.260.08417.1/255.1[M − H]
Isoliquiritin0.999130.38–1944.030.180.05417.1/255.1[M − H]
Liquiritigenin0.99952.12–135.550.610.18256.2/135.0[M − H]
Isoliquiritigenin0.99980.61–39.010.480.14256.2/135.0[M − H]