Comparative Study of Novel Ratio Spectra and Isoabsorptive Point Based Spectrophotometric Methods: Application on a Binary Mixture of Ascorbic Acid and Rutin

Table 1

Determination of AA and RU in laboratory prepared mixtures by the proposed spectrophotometric methods.

Concentration		Direct	A_Sum	Iso_RD	A_Sum	Iso_RD	Iso_P	RD	DD	MCN	RS
(µg/mL)
AA	RU	RU	RU	RU	AA	AA	AA	AA	AA	AA	AA
8	4	104.69	99.40	100.13	101.22	101.06	91.20	106.37	102.40	104.64	102.84
8	8	105.01	99.50	97.78	99.24	99.75	98.71	104.74	103.43	103.86	103.47
12	8	99.18	100.48	101.62	99.72	98.49	98.48	101.91	100.21	99.99	101.32
8	12	103.75	98.67	99.36	98.64	99.75	96.20	101.58	100.12	101.00	100.49
12	12	99.60	99.12	102.50	98.95	98.41	96.93	101.36	99.91	100.61	100.75
16	12	98.63	100.16	97.77	101.26	100.75	91.02	99.04	98.02	99.58	98.09
20	12	99.94	99.35	102.37	101.20	101.79	90.86	99.39	98.48	100.21	98.68
8	16	106.27	98.91	98.53	99.10	95.94	97.47	103.69	102.69	103.84	102.56
12	16	101.07	101.57	96.02	98.80	100.11	96.03	102.66	102.07	103.03	101.77
20	16	100.31	99.06	97.19	99.92	97.34	96.12	103.34	102.77	104.19	102.93
8	20	106.58	99.55	99.12	99.35	102.21	96.33	101.45	101.15	101.51	100.54
20	20	99.18	101.56	99.72	100.35	100.13	96.94	103.40	102.56	104.00	102.76
Mean		102.02	99.78	99.34	99.81	99.65	95.52	102.41	101.15	102.21	101.35
SD		2.88	0.97	2.05	0.98	1.83	2.73	2.00	1.71	1.81	1.64
RSD%		2.82	1.21	2.06	0.98	1.84	2.86	1.95	1.69	1.77	1.62

Average of four determinations.