High-Throughput Analysis of Lidocaine in Pharmaceutical Formulation by Capillary Zone Electrophoresis Using Multiple Injections in a Single Run

Table 1

Performance parameters of the method by CZE for analysis of lidocaine.

Parameters
Number of plates (N m^−1)	67.000–100.000
Resolution (range for neighboring peaks)	2.00–2.5
Linearity–calibration range (mg L^−1)^1	10–50
Linearity–slope (L mg^−1)^1	0.0186
Slope standard deviation^1	3.9 × 10^−4
Linearity–intercept^1	0.006
Intercept standard deviation^1	0.0002
	0.999
	7858
LOD (mg L^−1)^2	0.92
LOQ (mg L^−1)^2	3.1
Instrumental precision, RSD (%)–peak area^3	1.2–2.8 (1.0–3.0)
Instrumental precision, RSD (%)–migration time^3	0.51–0.55 (0.4–1.3)
Intra-day precision, RSD (%)–peak area^4	2.7–2.9 (2.5–3.5)
Intra-day precision, RSD (%)–migration time^4	0.25–1.2 (0.68–1.6)
Intermediate precision, RSD (%)–peak area^4	2.6–3.2
Intermediate precision, RSD (%)–migration time^4	0.83–0.95
Recovery (%)^5, added 8.0 mg L^−1, found 8.2 mg L^−1	102
Recovery (%)^5, added 16.0 mg L^−1, found 14.7 mg L^−1	92
Recovery (%)^5, added 24.0 mg L^−1, found 23.5 mg L^−1	98

Six levels of concentration () genuine replicates, injected in triplicate. ²LOD and LOQ calculated using the equations and LOQ = (10 × s)/S, where is the intercept standard deviation and is the slope of the analytical curve equation, respectively. ³Values referents to a standard and a sample, outside and inside the brackets, respectively. RSD range relative to four peaks injected (P1, P2, P3, and P4) (). ⁴Values referents to a standard and a sample, outside and inside the brackets, respectively. RSD range relative to three levels of concentration tested (). ⁵Recovery for an ointment sample.