Analysis of E. rutaecarpa Alkaloids Constituents In Vitro and In Vivo by UPLC-Q-TOF-MS Combined with Diagnostic Fragment

Table 1

Characterization of chemical constituents of Evodia by UPLC-Q-TOF-MS.

Number	RT	Identification	Formula	Theoretical mass ()	Experimental mass ()	Error; (ppm)	Blood components	MS data(+ ) ()
1	3.07	14-Formyldihydroxyrutaecarpine	C19H15N3O2	318.1243	318.1245	0.63	Y	273, 261, 217, 171, 144
2	4.29	Dehydroevodiamine	C19H15N3O	302.1295	302.1309	4.63	Y	302, 286, 261, 217, 177, 133
3	6.97	Evodiamine	C19H17N3O	304.1450	304.1439		Y	304, 171, 161, 144, 134, 116, 106
4	7.13	Rutaecarpine	C18H13N3O	288.1137	288.1141	1.39	Y	288, 273, 171, 169, 120
5	6.81	Evodiamide	C19H21N3O	308.1763	308.1753		N	308, 175, 165, 144, 134
6	7.87	1-Methyl-2-nonyl-4(1H)-quinolone	C19H27NO	286.2171	286.2171	0	N	286, 261, 217, 186, 173, 144, 102
7	8.20	1-Methyl-2-[(Z)-6-undecenyl]-4(1H)-quinolone/1-methyl-2-[(Z)-5-undecenyl]-4(1H)-quinolone	C21H29NO	312.2327	312.2334	2.24	N	312, 217, 186, 173, 144
8	8.31	1-Methyl-2-[(Z)-6-undecenyl]-4(1H)-quinolone/1-methyl-2-[(Z)-5-undecenyl]-4(1H)-quinolone	C21H29NO	312.2327	312.2321		N	312, 217, 186, 173, 144
9	8.69	1-Methyl-2-[(4Z,7Z)-4,7-tridecadienyl]-4(1H)-quinolone	C23H31NO	338.2484	338.2480		N	338, 217, 186, 173,
10	8.95	1-Methyl-2-undecyl-4(1H)-quinolone	C21H31NO	314.2484	314.2484	0	N	314, 186, 173, 144, 132
11	9.24	Evocarpine	C23H33NO	340.2640	340.2642	0.59	Y	340, 187, 186, 174, 173, 159, 144, 132
12	9.58	1-Methyl-2-dodecyl-4(1H)-quinolone	C22H33NO	328.2640	328.2633		N	328, 186, 173
13	9.63	1-Methyl-2-[(6Z,9Z)-6,9-pentadecadienyl]-4(1H)-quinolone	C25H35NO	366.2797	366.2814	4.64	N	354, 328, 305, 261, 217, 186, 173, 144
14	10.25	Dihydroevocarpine	C23H35NO	342.2797	342.2805	2.34	Y	342, 340, 261, 217, 186, 173, 144, 122
15	10.47	1-Methyl-2-[(Z)-9-pentadecenyl]-4(1H)-quinolone/1-methyl-2-[(Z)-10-pentadecenyl]-4(1H)-quinolone	C25H37NO	368.2953	368.2942		N	368, 342, 217, 186, 173, 144, 122
16	10.54	1-Methyl-2-[(Z)-9-pentadecenyl]-4(1H)-quinolone/1-methyl-2-[(Z)-10-pentadecenyl]-4(1H)-quinolone	C25H37NO	368.2953	368.2949		N	368, 342, 217, 186, 173, 144, 122
17	11.83	1-Methyl-2-pentadecyl-4(1H)-quinolone	C25H39NO	370.3110	370.3105		N	370, 261, 217, 186, 173, 144, 122

(a) Y represents detected in the blood and N denotes not detect.