Quantitative Clinical Diagnostic Analysis of Acetone in Human Blood by HPLC: A Metabolomic Search for Acetone as Indicator

Table 1

Quantitative analysis data of acetone as its 2,4-dinitrophenylhydrazone derivative in human blood by HPLC.

Sample	Subject	Sex	Age	Blood glucose	Urine ketone	Acetone levels by HPLC (mean value ± sd.)
						Blood (mmol L^−1)
Group 1
1	Type 1 diabetes mellitus, Hashimoto disease, systemic lupus erythematosus	F	21	197	++	15.00 ± 1.00
2	Diabetes mellitus (DM), pneumonia	M	68	320	+	2.40 ± 0.20
3	DM, obesity, depression	F	33	196	+	3.86 ± 0.10
4	Acute pancreatitis	M	60	134	+	0.013 ± 0.001
5	Acute pancreatitis, obesity, hyperlipidemia	F	33	117	+	0.22 ± 0.01
6	DM	F	37	175	+	0.24 ± 0.01
7	Gestational DM	F	37	74	+++	17.27 ± 1.00
Group 2
8	DM, hypothyroidemia	M	80	340	−	0.42 ± 0.01
9	DM, hypertension	F	82	248	−	3.20 ± 0.10
10	DM, hypertension, cerebrovascular disease, acute kidney failure	F	77	202	−	3.10 ± 0.10
11	Anemia, hypothyroidemia, hyperlipidemia	M	52	110	−	0.033 ± 0.01
12	DM, hypertension, hyperlipidemia	F	55	137	−	1.53 ± 0.01
13	Hypertension, congestive heart disease, chronic obstructive pulmonary disease, cerebrovascular disease	M	83	182	−	1.51 ± 0.01
14	DM, larynx carcinoma, hypothyroidemia	M	59	177	−	1.54 ± 0.01
15	Hepatic failure, hypertension, chronic kidney failure	M	87	111	−	2.13 ± 0.10

Number of repeated experiments, , and reference value: 0.034–0.120 mmol L⁻¹ with a mean value in plasma: 0.075 mmol L⁻¹ () [2].