Assessment for Melting Temperature Measurement of Nucleic Acid by HRM

Figure 1

Fluorescence melting curves (a) and differential curves (b) of 6–20 bp DNA duplexes. Each DNA duplex (1 μM) was measured in a 10 μL solution containing 1x EvaGreen, 10 mM phosphate (pH 7.4), and 100 mM NaCl.

(a)

(b)