Assessment for Melting Temperature Measurement of Nucleic Acid by HRM

Figure 4

Effect of Na⁺ or Mg²⁺ on measurement by HRM. s of DNA duplex GC3/15 and GC12/15 were measured by HRM with NaCl at various concentrations from 0 to 1 M (a) or with MgCl₂ from 1 to 100 mM (b). The corresponding s were measured by UV.

(a)

(b)