Assessment for Melting Temperature Measurement of Nucleic Acid by HRM

Figure 6

measurement of DNA triplex by HRM (a) and by UV melting method (b). DNA duplex (black) and triplex (red) were detected in the same buffer solution (1 μM of each strand, 2.5 mM KCl, 10 mM Na₂HPO₄, 2 mM KH₂PO₄, 10 mM MgCl₂, and 0.5 mM spermine (pH 5.5)).

(a)

(b)