Research Article

Assessment for Melting Temperature Measurement of Nucleic Acid by HRM

Table 1

Sequences of DNA and RNA duplexes used in this study.

NameSequence of duplex (5′-F strand-3′/3′-R strand-5′)

L6CTATCC/GATAGG
L7CTATCAC/GATAGTG
L8CGTATCAC/GCATAGTG
L9CGTCATCAC/GCAGTAGTG
L10CGTCATCAGC/GCAGTAGTCG
L15GCCCTGGTGATTAAA/CGGGACCACTAATTT
L20GGGTGCCGTATTGACAAAAC/CCCACGGCATAACTGTTTTG
GC0/15TAAAATAATAATAAT/ATTTTATTATTATTA
GC1/15TATAAATAAGTAAAT/ATATTTATTCATTTA
GC2/15AAGTACATTTATATA/TTCATGTAAATATAT
GC3/15AATAAACATTCTCAA/TTATTTGTAAGAGTT
GC4/15GTTTTTCAGTGAATA/CAAAAAGTCACTTAT
GC5/15GGTTTTTCAGTGAAT/CCAAAAAGTCACTTA
GC6/15AGAGGTTTTTCAGTG/TCTCCAAAAAGTCAC
GC7/15GCCCTGGTGATTAAA/CGGGACCACTAATTT
GC8/15CTCACGCCTGTAATC/GAGTGCGGACATTAG
GC9/15GAGTCTCGCTCTGTC/CTCAGAGCGAGACAG
GC10/15TGGCACCGAGGTGAC/ACCGTGGCTCCACTG
GC11/15TGCGTGGCACCGAGG/ACGCACCGTGGCTCC
GC12/15GAGGTGCACCGCCGC/CTCCACGTGGCGGCG
GC13/15CGGCGCCCTCGCTCC/GCCGCGGGAGCGAGG
GC14/15GCCGCGGCGCCCTCG/CGGCGCCGCGGGAGC
GC15/15GGCCGGCCGCGGCGC/CCGGCCGGCGCCGCG
GC0/20AATAATAATAATAATAATAT/TTATTATTATTATTATTATA
GC2/20ATTTTCTATTTTTTTAACTT/TAAAAGATAAAAAAATTGAA
GC4/20AAAAACAGAAGTAAGATAAT/TTTTTGTCTTCATTCTATTA
GC6/20CCGTATTGACAAAACATTAA/GGCATAACTGTTTTGTAATT
GC8/20TGTCCTTCCGAGTATGATAT/ACAGGAAGGCTCATACTATA
GC10/20GGGTGCCGTATTGACAAAAC/CCCACGGCATAACTGTTTTG
GC12/20GAGGGAGCAGGAAGATCCGT/CTCCCTCGTCCTTCTAGGCA
GC14/20GGAAGATCCGTGCGGCACCG/CCTTCTAGGCACGCCGTGGC
GC16/20GCGCCCTCGCTCCTCGCCCT/CGCGGGAGCGAGGAGCGGGA
GC18/20GGCCGCGGCGCCCTCGCTCC/CCGGCGCCGCGGGAGCGAGG
GC20/20CGGGGCCGGCCGCGGCGCCC/GCCCCGGCCGGCGCCGCGGG
RRCUGACCUAUGAAUUGACAGCC/GACUGGAUACUUAACUGUCGG