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Journal of Analytical Methods in Chemistry
Volume 2016, Article ID 7201740, 5 pages
Research Article

Enrichment of the Glycyrrhizic Acid from Licorice Roots (Glycyrrhiza glabra L.) by Isoelectric Focused Adsorptive Bubble Chromatography

1Department of Forest Industry Engineering, Faculty of Forestry, Kahramanmaraş Sutcu Imam University, Kahramanmaraş, Turkey
2Faculty of Health Sciences, Istanbul Esenyurt University, Esenyurt, 34510 Istanbul, Turkey
3Department of Chemical-Technical Analysis and Chemical Food Technology, Technical University of Munich, Munich, Germany

Received 28 October 2015; Revised 16 December 2015; Accepted 17 December 2015

Academic Editor: Hassan Y. Aboul Enein

Copyright © 2016 Eyyüp Karaoğul et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The main aim of this study was to enrich glycyrrhizic acid ammonium salt known as one of the main compounds of licorice roots (Glycyrrhiza glabra L.) by isoelectric focused adsorptive bubble separation technique with different foaming agents. In the experiments, four bubble separation parameters were used with β-lactoglobulin, albumin bovine, and starch (soluble) preferred as foaming agents and without additives. The enrichment of glycyrrhizic acid ammonium salt into the foam was influenced by different additive substances. The results showed that highest enrichment values were obtained from β-lactoglobulin as much as 368.3 times. The lowest enrichment values (5.9 times) were determined for the application without additive. After enrichment, each experiment of glycyrrhizic acid ammonium salt confirmed that these substances could be quantitatively enriched into the collection vessel with isoelectric focused adsorptive bubble separation technique. The transfer of glycyrrhizic acid ammonium salt into the foam from standard solution in the presence of additive was more efficient than aqueous licorice extract.