Research Article

Capitalizing Resolving Power of Density Gradient Ultracentrifugation by Freezing and Precisely Slicing Centrifuged Solution: Enabling Identification of Complex Proteins from Mitochondria by Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Figure 2

Comparison of distribution of mitochondrial complex V in three adjacent aliquots (sucrose density 34–43%) by BN-PAGE. (a) BN-PAGE results of aliquots fractionated by pipetting method. (b) BN-PAGE results of aliquots fractionated by F-S method.
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