Extraction Efficiency of Different Solvents and LC-UV Determination of Biogenic Amines in Tea Leaves and Infusions

Table 2

LC-UV method performance ().

BA	Extraction solvents
	HCl 0.1 M							HClO4 0.1 M							TCA 5% (w/w)
		LOD; (μg L^−1)	LOQ; (μg L^−1)	LOD; (μg g^−1)	LOQ; (μg g^−1)	LOD; (μg L^−1)	LOQ; (μg L^−1)		LOD; (μg L^−1)	LOQ; (μg L^−1)	LOD; (mg Kg^−1)	LOQ; (mg Kg^−1)	LOD; (μg L^−1)	LOQ; (μg L^−1)		LOD; (μg L^−1)	LOQ; (μg L^−1)	LOD; (mg Kg^−1)	LOQ; (mg Kg^−1)	LOD; (μg L^−1)	LOQ; (μg L^−1)
PHE	0.99	0.29	0.91	0.41	1.08	0.31	0.92	0.99	0.30	0.90	0.40	1.11	0.32	0.95	0.99	0.27	0.90	0.40	1.07	0.30	0.90
PUT	0.99	0.11	0.30	0.25	0.80	0.18	0.52	0.99	0.10	0.32	0.27	0.82	0.19	0.53	0.99	0.10	0.29	0.23	0.70	0.18	0.50
CAD	0.99	0.19	0.60	0.35	1.09	0.32	0.98	0.99	0.20	0.58	0.36	1.10	0.33	0.99	0.99	0.17	0.59	0.33	1.08	0.30	0.97
HIS	0.99	0.30	0.92	0.40	1.03	0.32	0.95	0.99	0.28	0.90	0.41	1.05	0.33	0.97	0.99	0.29	0.90	0.35	1.00	0.31	0.94
TYR	0.99	0.41	1.28	0.45	1.31	0.43	1.30	0.99	0.42	1.26	0.46	1.35	0.45	1.33	0.99	0.40	1.27	0.40	1.30	0.42	1.28
SPD	0.99	0.27	0.72	0.32	0.83	0.29	0.76	0.99	0.29	0.70	0.31	0.82	0.30	0.78	0.99	0.26	0.70	0.30	0.80	0.27	0.75
SER	0.99	0.24	0.78	0.30	0.90	0.26	0.84	0.99	0.26	0.75	0.32	0.92	0.27	0.85	0.99	0.23	0.77	0.29	0.88	0.25	0.83
SPM	0.99	0.25	0.60	0.27	0.75	0.30	0.81	0.99	0.24	0.61	0.29	0.76	0.32	0.86	0.99	0.24	0.59	0.24	0.70	0.29	0.80

TYR was added (100 μL 0.05 mg mL⁻¹); BA standard solution; tea leaves; tea infusion. PHE, β-phenylethylamine; PUT, putrescine; CAD, cadaverine; HIS, histamine; TYR, tyramine; SPD, spermidine; SER, serotonin; SPM, spermine. TCA, trichloroacetic acid, LOD, limit of detection; LOQ, limit of quantitation.