Extraction Efficiency of Different Solvents and LC-UV Determination of Biogenic Amines in Tea Leaves and Infusions
Table 3
Values of recovery, coefficient of variation, and intra- and interday repeatability obtained with LC-UV on tea leaves.
BA
HCl 0.1 M
HClO4 0.1 M
TCA 5% (w/w)
Recovery (%)
CV
RSD intraday
RSD interday
Recovery (%)
CV
RSD intraday
RSD interday
Recovery (%)
CV
RSD intraday
RSD interday
PHE
90.4
6.8
0.3
0.4
91.4
6.9
0.3
0.4
98.4
4.7
0.2
0.3
PUT
92.3
4.1
0.3
0.4
88.3
4.1
0.3
0.4
98.3
4.1
0.1
0.2
CAD
94.0
4.4
0.3
0.4
94.2
4.5
0.3
0.4
96.5
1.4
0.1
0.2
HIS
92.5
4.9
0.3
0.4
87.5
4.3
0.3
0.4
97.5
2.4
0.2
0.3
TYR
91.0
—
0.2
0.3
89.0
—
0.2
0.3
98.0
—
0.1
0.2
SPD
89.5
4.6
0.4
0.4
88.5
4.9
0.3
0.4
98.5
2.4
0.1
0.2
SER
88.0
6.0
0.4
0.4
87.8
6.1
0.4
0.4
98.9
1.9
0.1
0.2
SPM
94.0
6.0
0.3
0.4
93.0
4.8
0.3
0.4
98.0
2.4
0.2
0.3
2.0 g of sample 5 was spiked with 1.0 mL of a BAs standard solution at concentration of 2.0 mg L−1. PHE, β-phenylethylamine; PUT, putrescine; CAD, cadaverine; HIS, histamine; TYR, tyramine; SPD, spermidine; SER, serotonin; SPM, spermine; TCA: trichloroacetic acid.
