Journal of Analytical Methods in Chemistry

Research Article

Extraction Efficiency of Different Solvents and LC-UV Determination of Biogenic Amines in Tea Leaves and Infusions

Table 4

Values of recovery, coefficient of variation, and intra- and interday repeatability obtained with LC-UV on tea infusions.


AB	HCl 0.1 M				HClO₄ 0.1 M				TCA 5% (w/w)
AB	Recovery (%)	CV	RSD intraday	RSD interday	Recovery (%)	CV	RSD intraday	RSD interday	Recovery (%)	CV	RSD intraday	RSD interday

PHE	90.4	—	0.3	0.4	91.4	—	0.3	0.4	97.4	—	0.1	0.3
PUT	92.3	2.7	0.3	0.4	88.3	2.6	0.3	0.4	98.8	2.6	0.1	0.2
CAD	93.0	6.2	0.3	0.4	94.5	6.4	0.3	0.4	97.5	2.2	0.2	0.3
HIS	90.5	4.3	0.3	0.3	87.5	4.5	0.3	0.3	97.6	2.2	0.1	0.2
TYR	90.0	—	0.2	0.3	88.0	—	0.2	0.3	99.0	—	0.1	0.2
SPD	88.5	5.8	0.4	0.4	88.5	6.0	0.4	0.4	97.5	2.8	0.1	0.2
SER	87.0	10.0	0.4	0.5	86.8	9.5	0.4	0.5	99.9	3.5	0.1	0.2
SPM	94.1	7.8	0.3	0.4	95.0	6.6	0.3	0.4	98.0	4.8	0.1	0.2

40 mL of sample 5 was spiked with 5.0 mL of a BAs standard solution at concentration of 2.0 mg L⁻¹.
PHE, β-phenylethylamine; PUT, putrescine; CAD, cadaverine; HIS, histamine; TYR, tyramine; SPD, spermidine; SER, serotonin; SPM, spermine; TCA: trichloroacetic acid.