Preparation of a Specific ssDNA Aptamer for Brevetoxin-2 Using SELEX

Figure 1

Identification of dsDNA and ssDNA using electrophoresis. (a) 2% sepharose gel electrophoresis of the 85-bp dsDNA amplified by symmetric PCR. Lane 1 is the 85-bp dsDNA, and lane 2 is the DL2000 DNA Marker; (b) 8% denaturing urea gel electrophoresis of ssDNA. Lane 1 is the DL2000 DNA Marker, and lane 2 is the 85-nt ssDNA amplified by asymmetric PCR.

(a)

(b)