Preparation of a Specific ssDNA Aptamer for Brevetoxin-2 Using SELEX

Figure 4

Binding activity and cross-reactivity of Bap5. (a) The value of the Bap5 aptamer was measured using different BTX-2-OVA coating concentrations (1, 0.5, and 0.25 μg mL⁻¹) to estimate the dissociation constant (). The -axis represents the aptamer concentration (ng mL⁻¹), whereas the -axis represents the absorbance at 492 nm. (b) Plots from the ELISA experiments used to investigate the specificity of the Bap5 aptamer for BTX-2 and other toxins. The calibration curve was obtained with the inhibition percent shown on the -axis and the concentration of toxins on the -axis. is the OD₄₉₂ value when the toxin standard is not present in the detected sample (control), and is the OD₄₉₂ value when there is a difference in the concentration of the standard in the detected sample. All the data represent the means ± SD () of three replicates.

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