Preparation of a Specific ssDNA Aptamer for Brevetoxin-2 Using SELEX

Figure 5

Plot of the indirect competitive ELISA of the BTX-2 aptamer Bap5. The calibration curve was obtained with the inhibition percent on the -axis and the log concentration of BTX-2 on the -axis. The and values were the OD₄₉₂ of the control and the sample, respectively. As the concentration of the BTX-2 standard increased (ranging from 3.125 to 200 ng mL⁻¹), the inhibition ratio ranged from 11.67% to 67.3%.