Immobilization of NTPDase-1 from Trypanosoma cruzi and Development of an Online Label-Free Assay

Figure 1

Representative scheme of enzyme immobilization via Schiff’s base formation. Capillary support was activated with 3-aminopropyltrietoxysilane (APTS) and glutaraldehyde, subsequently N-terminal amino group of enzyme binding to aldehyde support.