Immobilization of NTPDase-1 from Trypanosoma cruzi and Development of an Online Label-Free Assay

Figure 3

Representative chromatogram for the separation of ATP (3), ADP (2), and AMP (1) by a Phenomenex Luna C18 (100 Å 250 × 4.6 mm, 5 μm) analytical column at a flow rate of 1.0 mL⋅min⁻¹, eluent Buffer C : MeOH (84 : 16 v/v), and UV detection at λ = 254 nm. The chromatographic conditions of the two-dimensional method are described in Table 1.