Immobilization of NTPDase-1 from Trypanosoma cruzi and Development of an Online Label-Free Assay

Figure 4

(—) Representative chromatogram of the separation of ATP (2) and ADP (1) by a Luna C18 analytical column. (- - -) Representative chromatogram of the activity of the immobilized enzyme TcNTPDase-1-ICER. On-line hydrolysis of ATP (10 μL of 1.0 mmol⋅L⁻¹) to ADP. Separation obtained by a Luna C18 analytical column coupled to the TcNTPDase-1-ICER, max = 254 nm. Experimental conditions as described in Table 1.