Research Article
Immobilization of NTPDase-1 from Trypanosoma cruzi and Development of an Online Label-Free Assay
Table 1
Chromatographic conditions used in the TcNTPDase-1-ICER two-dimensional method.
| | Pump | Time (min) | Valve position | Event |
| | A | 0.0–0.3 | 1 | Enzymatic reaction in TcNTPDase-1-ICER | | B | 0.0–0.5 | 1 | Analytical column conditioning | | A | 0.31–8.0 | 2 | Analyte transfer to the analytical column | | A | 8.01–20.0 | 1 | TcNTPDase-1-ICER conditioning | | B | 8.01–20.0 | 1 | Analytical separation of the analytes in the second dimension |
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Pump A: flow rate: 0.03 mL⋅min−1, eluent: Buffer A. Pump B: flow rate: 1.0 mL⋅min−1, eluent: Buffer C : MeOH (84 : 16; v/v).
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