Research Article
UHPLC-PDA Assay for Simultaneous Determination of Vitamin D3 and Menaquinone-7 in Pharmaceutical Solid Dosage Formulation
Table 1
Results of tested stationary phase.
| Column | Analyte | | | | |
| HPLC | Hypersil ODS (250 × 4.6 mm, 5 μm) | Vitamin D3 | — | 1.11 | — | 4132 | MK-7 | 13.14 | 1.36 | 5.64 | 8329 | Venusil XBP C18 (250 × 4.6 mm, 5 μm) | Vitamin D3 | — | 1.14 | — | 3763 | MK-7 | 12.54 | 1.31 | 5.31 | 7296 | ACE 5 C18 (250 × 4.6 mm, 5 μm) | Vitamin D3 | — | 1.11 | — | 5867 | MK-7 | 15.92 | 1.12 | 6.83 | 9683 | Purespher® RP-18 (250 × 4.6 mm, 5 μm) | Vitamin D3 | — | 1.37 | — | 3923 | MK-7 | 13.45 | 1.09 | 5.91 | 8209 |
| UHPLC | ACE Excel 2 C18-PFP (100 × 2.1 mm, 2 μm) | Vitamin D3 | — | 0.94 | — | 23289 | MK-7 | 27.61 | 0.98 | 4.61 | 28521 | Waters ACQUITY 1.7 BEH C 18 (100 × 2.1 mm, 2 μm) | Vitamin D3 | — | 1.32 | — | 19342 | MK-7 | 24.23 | 1.15 | 4.25 | 25029 | Agilent Poroshell 2.7 120 EC C18 (100 × 2.1 mm, 2 μm) | Vitamin D3 | — | 1.23 | — | 15687 | MK-7 | 21.54 | 1.15 | 3.71 | 22143 | Phenomenex Kinetex 2.6 C18 (100 × 2.1 mm, 2 μm) | Vitamin D3 | — | 1.07 | — | 11981 | MK-7 | 19.21 | 1.12 | 3.31 | 19814 |
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