UHPLC-PDA Assay for Simultaneous Determination of Vitamin D3 and Menaquinone-7 in Pharmaceutical Solid Dosage Formulation
Table 4
Accuracy studies of vitamin D3 and MK-7 by HPLC and UHPLC.
Analyte
Concentration after spiking (μg mL−1)
HPLC
UHPLC
-experimental -experimental
Concentration found (μg mL−1) ± SEM; RSD
(%) recovery BIAS
Concentration found (μg mL−1) ± SEM; RSD
(%) recovery BIAS
Vitamin D3
37.5
37.53 ± 0.21; 1.18
100.08
37.55 ± 0.22; 1.48
100.13
0.64 4.4
0.08
0.13
50.0
50.87 ± 0.18; 0.71
101.74
50.78 ± 0.21; 0.71
101.56
−4.16 8.7
1.74
1.56
62.5
62.48 ± 0.17; 0.43
99.97
62.57 ± 0.14; 0.33
100.11
−1.15 0.79
0.03
0.11
MK-7
37.5
37.12 ± 0.22; 2.08
98.97
37.26 ± 0.32; 2.55
99.36
0.52 0.55
1.01
0.64
50.0
50.56 ± 0.21; 0.98
101.12
50.58 ± 0.42; 1.16
101.16
−0.29 0.46
1.12
1.16
62.5
62.79 ± 0.24; 1.98
100.46
62.27 ± 0.51; 0.40
99.63
5.77 0.32
0.46
0.83
Actual concentration of vitamin D3 and MK-7 = 25 μg mL−1. All measurements were made in triplicate; SEM: standard error mean; RSD: relative standard deviation. Theoretical -value is 4.30 and -value is 19.0, at = 0.05.
