Development and Validation of a Stability-Indicating HPLC Method for the Simultaneous Determination of Florfenicol and Flunixin Meglumine Combination in an Injectable Solution

<table class="table-group" id="tab2"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td class="align_left">Stress type</td><td class="align_center">Conditions</td><td class="align_center">Time</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td class="align_left">Acid hydrolysis</td><td class="align_center">1 N HCl; at 40°C</td><td class="align_center">2 days</td></tr><tr><td class="align_left">Base hydrolysis</td><td class="align_center">0.02 N NaOH; at RT</td><td class="align_center">2 hours</td></tr><tr><td class="align_left">Oxidative/solution</td><td class="align_center">0.2% H<sub>2</sub>O<sub>2</sub> at 40°C;<br/> protected from light</td><td class="align_center">7 days</td></tr><tr><td class="align_left">Thermal</td><td class="align_center">75°C</td><td class="align_center">14 days</td></tr><tr><td class="align_left">Photodegradation</td><td class="align_center">UV light</td><td class="align_center">3 days</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Stress conditions applied for drug substance and drug product.

Journal of Analytical Methods in Chemistry

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Table 2

Table 2: Development and Validation of a Stability-Indicating HPLC Method for the Simultaneous Determination of Florfenicol and Flunixin Meglumine Combination in an Injectable Solution 

Table 2 | Development and Validation of a Stability-Indicating HPLC Method for the Simultaneous Determination of Florfenicol and Flunixin Meglumine Combination in an Injectable Solution 