Spectrophotometric Methods for Simultaneous Determination of Oxytetracycline HCl and Flunixin Meglumine in Their Veterinary Pharmaceutical Formulation

Table 3

Determination of Oxytetracycline HCl and Flunixin Meglumine in FLOXON by the proposed dual wavelength and first-derivative spectrophotometric methods and application of standard addition technique.

Method	Drug	Found (%) ± SD	Standard addition technique
			Pure added; (µg/mL)	Found; (µg/mL)	Recovery %
Dual wavelength	OXY	103.87 ± 1.75	5	5.07	101.40
			25	25.07	100.28
			30	30.40	101.33
	Mean ± RSD				101.00 ± 0.62
	FLU	97.14 ± 1.63	5	4.99	99.80
			10	10.08	100.80
			15	15.28	101.87
	Mean ± RSD				100.82 ± 1.03
First derivative	OXY	103.76 ± 0.58	5	5.03	100.60
			25	24.89	99.56
			30	30.03	100.10
	Mean ± RSD				100.09 ± 0.52
	FLU	98.07 ± 1.00	5	4.91	98.20
			10	9.81	98.10
			15	14.85	99.00
	Mean ± SD				98.43 ± 0.50

Average of three determinations of FLOXON tables (labeled to contain 108 mg/mL Oxytetracycline HCl and 33 mg/mL Flunixin Meglumine), BN 160142.