Spectrophotometric Methods for Simultaneous Determination of Oxytetracycline HCl and Flunixin Meglumine in Their Veterinary Pharmaceutical Formulation

Table 4

Determination of Oxytetracycline HCl and Flunixin Meglumine in FLOXON by the proposed ratio difference and first derivative of ratio spectra spectrophotometric methods and application of standard addition technique.

Method	Drug	Found (%) ± SD	Standard addition technique
			Pure added; (µg/mL)	Found; (µg/mL)	Recovery %
Ratio difference	OXY	102.40 ± 1.06	5	4.95	99.00
			25	24.82	99.28
			30	29.93	99.77
	Mean ± RSD				99.35 ± 0.39
	FLU	97.50 ± 1.23	5	4.92	98.40
			10	10.04	100.40
			15	15.13	100.87
	Mean ± RSD				99.89 ± 1.31
Derivative ratio	OXY	102.74 ± 1.07	5	5.09	101.80
			25	25.31	101.24
			30	30.55	101.83
	Mean ± RSD				101.62 ± 0.33
	FLU	96.63 ± 1.43	5	5.01	100.20
			10	10.09	100.90
			15	15.12	100.80
	Mean ± SD				100.63 ± 0.38

Average of three determinations of FLOXON tables (labeled to contain 108 mg/mL Oxytetracycline HCl and 33 mg/mL Flunixin Meglumine), BN 160142.