Journal of Analytical Methods in Chemistry / 2017 / Article / Fig 3

Research Article

Stereospecific Assay of (R)- and (S)-Goitrin in Commercial Formulation of Radix Isatidis by Reversed Phase High-Performance Liquid Chromatography

Figure 3

Effect of (a) methanol concentration, (b) flow rate, and (c) column temperature on the retention time of (R)-goitrin (▲) and (S)-goitrin (■). Experimental conditions: S-Chiral A column (4.6 mm × 250 mm, 5 μm), methanol/water, 245 nm, and injection volume of 10 μL.
(a)
(b)
(c)

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