Stereospecific Assay of (R)- and (S)-Goitrin in Commercial Formulation of Radix Isatidis by Reversed Phase High-Performance Liquid Chromatography

Figure 4

Typical chromatograms obtained for the blank reference, standard solution containing racemic (R, S)-goitrin, and commercial granules. Experimental conditions: S-Chiral A column (4.6 mm × 250 mm, 5 μm), methanol/water (30 : 70, v/v), 0.4 mL/min, 35°C, 245 nm, and injection volume of 10 μL.