Stereospecific Assay of (R)- and (S)-Goitrin in Commercial Formulation of Radix Isatidis by Reversed Phase High-Performance Liquid Chromatography

Table 1

Optimized conditions for (R, S)-goitrin enantiomer separation.

Parameter	Optimization result
Stationary phase	S-Chiral A column (4.6 mm × 250 mm, 5 μm)
Column temperature	35°C
Mobile phase	Methanol/water (30 : 70, v/v)
Flow rate	0.4 mL/min
Detection wavelength	245 nm
Injection volume	10 μL