Research Article
Stereospecific Assay of (R)- and (S)-Goitrin in Commercial Formulation of Radix Isatidis by Reversed Phase High-Performance Liquid Chromatography
Table 1
Optimized conditions for (R, S)-goitrin enantiomer separation.
| Parameter | Optimization result |
| Stationary phase | S-Chiral A column (4.6 mm × 250 mm, 5 μm) | Column temperature | 35°C | Mobile phase | Methanol/water (30 : 70, v/v) | Flow rate | 0.4 mL/min | Detection wavelength | 245 nm | Injection volume | 10 μL |
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