Journal of Analytical Methods in Chemistry / 2017 / Article / Tab 1

Research Article

Stereospecific Assay of (R)- and (S)-Goitrin in Commercial Formulation of Radix Isatidis by Reversed Phase High-Performance Liquid Chromatography

Table 1

Optimized conditions for (R, S)-goitrin enantiomer separation.

ParameterOptimization result

Stationary phaseS-Chiral A column (4.6 mm × 250 mm, 5 μm)
Column temperature35°C
Mobile phaseMethanol/water (30 : 70, v/v)
Flow rate0.4 mL/min
Detection wavelength245 nm
Injection volume10 μL

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