Simultaneous Analysis of Ursolic Acid and Oleanolic Acid in Guava Leaves Using QuEChERS-Based Extraction Followed by High-Performance Liquid Chromatography

Table 1

Validation data for the target analytes in guava leaves.

Analyte	Concentration range (μg mL^−1)	Calibration curve	LOD (μg mL^−1)	LOQ (μg mL^−1)	Spiked level (μg mL^−1)	Precision, RSD%		Relative recovery, % (RSD, %) ()
						Intraday ()	Interday ()
Oleanolic acid	1–160		0.36	1.2	12	1.95	2.55	109.0 (0.38)
					24			97.4 (3.32)
					36			108.2 (3.18)
Ursolic acid	2–320		0.18	0.6	60	1.48	1.18	101.6 (4.10)
					120			99.2 (2.53)
					180			111.4 (7.14)