Analytical Method for the Validation of Three Polyphenols as a Marker Compound for the Standardization of Solidago virgaurea subsp. gigantea Extracts and Antiadipogenesis of Harvesting Time and Location

Table 1

Method validation parameters for quantitation of protocatechuic acid, chlorogenic acid, and kaempferol-3-O-rutinoside.

Parameters	Protocatechuic acid (PC)	Chlorogenic acid (CA)	Kaempferol-3-O-rutinoside (K-O-R)
Retention time (min; )
Regression equation
Correlation coefficient ()	1.0000	0.9999	0.9989
Linear range (μg/ml)	4.85–485.00	47.5–1900.00	8.50–850.00
LOQ (μg/ml)	0.630	9.358	4.360
LOD (μg/ml)	0.208	3.088	1.439

X: concentration of tested compounds (1)–(3); Y: peak area at 254 nm; LOQ: 10 × (standard deviation of the response/slope of calibration curve); LOD: 3.3 × (standard deviation of the response/slope of calibration curve).