Simultaneous Determination of 5 Flavonoids and 7 Saponins for Quality Control of Traditional Chinese Medicine Preparation Xinnaoshutong Capsule Using HPLC-VWD-ELSD

Figure 2

Typical HPLC-VWD-ELSD chromatograms for all of the reference compounds (a, b) and real sample (c, d). (1) Protodioscin, (2) (25R)-26-O-β-D-glucopyranosyl-5α-furostan-3β,22α,26-triol-3-O-β-D-xylopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-galactopyranoside, (3) Pseudoprotodioscin, (4) 26-O-β-D-glucopyranosyl-(25R)-5α-furostan-20(22)-en-3β,26-diol-3-O-β-D-xylopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-galactopyranoside}, (5) Terrestrinin A, (6) hecogenin-3-O-β-D-glucopyranosyl(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside, (7) Tibllin, (8) Rutin, (9) Isoquercitrin, (10) kaempferol, (11) quercetin, and (12) isorhamnetin.

(a)

(b)

(c)

(d)