Journal of Analytical Methods in Chemistry / 2017 / Article / Tab 2 / Research Article
Validation of an UHPLC-MS/MS Method for Screening of Antimicrobial Residues in Eggs and Their Application to Analyses of Eggs from Laying Hens Subjected to Pharmacological Treatment Table 2 MRM transitions and MS/MS parameters for tetracyclines, aminoglycosides, quinolones, and lincosamides after optimization, in the trichloroacetic acid extract.
Analytes DP Major transition CE Minor transition 1 (m/z ) CE Minor transition 2 CE RT Relative intensity Tetracyclines Chlortetracycline 61 479/98 67 479/275 55 — — 6.0 84.4 ± 5.0 Doxycycline 55 445/428 25 445/154 40 — — 6.3 5.8 ± 0.6 Oxytetracycline 41 461/201 59 461/283 53 — — 4.9 50.1 ± 4.1 Tetracycline 55 445/410 27 445/427 25 — — 5.2 25.9 ± 2.1 Aminoglycosides Amikacin 60 586/425 21 586/163 53 — — 4.8 374.9 ± 52.7 Apramycin 82 540/217 35 540/378 25 — — 5.2 112.6 ± 13.3 Dihydrostreptomycin 120 584/263 42 584/246 54 584/409 4 4.3 33.1 ± 3.2 Gentamicin 50 478/157 25 464/322 20 464/160 20 5.5 132.5 ± 20.1 Hygromycin 50 528/177 25 528/352 25 — — 4.2 207.4 ± 19.9 Kanamycin 70 485/163 35 485/205 35 — — 4.8 45.5 ± 5.7 Neomycin 120 615/161 41 615/296 35 — — 5.9 25.9 ± 2.7 Spectinomycin 66 351/207 31 351/189 33 — — 3.6 52.1 ± 2.6 Streptomycin 157 582/263 45 582/246 51 582/407 54 4.3 55.1 ± 7.4 Tobramycin 50 468/163 20 468/324 55 — — 5.3 7.4 ± 2.0 Quinolones Ciprofloxacin 61 332/314 30 332/231 47 — — 4.8 79.9 ± 12.1 Enrofloxacin 72 360/342 30 360/286 50 — — 5.2 28.2 ± 5.2 Flumequine 44 262/244 25 262/202 45 — — 7.0 9.6 ± 1.5 Nalidixic acid 42 233/215 30 233/187 35 — — 6.8 96.2 ± 2.9 Norfloxacin 60 320/302 33 320/231 50 — — 4.7 26.9 ± 4.2 Oxolinic acid 53 262/244 25 262/216 40 — — 5.4 17.1 ± 1.6 Sarafloxacin 50 386/368 30 386/348 40 — — 5.6 5.8 ± 2.2 Lincosamides Lincomycin 60 407/126 40 407/359 26 — — 4.3 8.2 ± 0.7
