Journal of Analytical Methods in Chemistry / 2017 / Article / Tab 3 / Research Article
Validation of an UHPLC-MS/MS Method for Screening of Antimicrobial Residues in Eggs and Their Application to Analyses of Eggs from Laying Hens Subjected to Pharmacological Treatment Table 3 MRM transitions and MS/MS parameters for β -lactams, sulfonamides, and macrolides after optimization, in the acetonitrile acid extract.
Analytes DP Major transitionm/z ) CE Minor transition 1 m/z ) CE Minor transition 2 m/z ) CE RT Relative intensity β -lactams Cefazolin 50 455/323 15 455/156 23 — — 4.1 53.6 ± 3.3 Cloxacillin 50 436/160 20 436/277 20 — — 8.3 120.5 ± 7.2 Dicloxacillin 50 470/160 20 470/311 20 — — 8.6 65.9 ± 4.2 Nafcillin 50 415/199 20 415/171 50 — — 8.4 31.4 ± 0.9 Oxacillin 50 402/160 18 402/243 18 — — 8.0 105.4 ± 9.3 Penicillin G 70 335/176 21 335/160 21 335/114 37 7.3 103.7 ± 11.2 Penicillin V 66 351/160 15 351/192 17 — — 7.7 9.9 ± 3.1 Sulfonamides Sulfachloropyridazine 51 285/156 21 285/92 39 — — 4.6 39.4 ± 1.8 Sulfadiazine 53 251/156 22 251/108 30 — — 2.6 39.2 ± 3.4 Sulfadimethoxine 50 311/156 23 311/108 37 — — 6.1 30.8 ± 1.5 Sulfadoxine 60 311/156 25 311/108 40 — — 5.0 30.8 ± 1.5 Sulfamerazine 60 265/92 35 265/156 35 — — 3.1 44.6 ± 3.0 Sulfamethazine 50 279/156 25 279/108 36 — — 3.6 76.8 ± 3.2 Sulfamethoxazole 60 254/108 35 254/92 35 — — 5.0 117.3 ± 9.5 Sulfamethoxypyridazine 60 281/156 25 281/108 35 — — 4.0 40.7 ± 2.0 Sulfaquinoxaline 50 301/156 23 301/108 40 — — 6.2 33.6 ± 1.3 Sulfathiazole 53 256/156 20 256/108 34 — — 3.1 33.6 ± 1.5 Sulfisoxazole 46 268/156 21 268/113 23 — — 5.4 59.8 ± 5.0 Macrolides Clindamycin 75 425/126 43 425/377 27 — — 6.9 8.9 ± 0.3 Erythromycin 66 734/158 43 734/576 27 — — 7.6 50.3 ± 2.0 Spiramycin 56 422/174 31 422/101 25 — — 7.4 65.7 ± 8.4 Tilmicosin 56 869/174 63 869/696 57 — — 7.8 61.1 ± 3.4 Tylosin 115 916/174 55 916/772 43 — — 7.8 35.1 ± 1.4
