Validation of an UHPLC-MS/MS Method for Screening of Antimicrobial Residues in Eggs and Their Application to Analyses of Eggs from Laying Hens Subjected to Pharmacological Treatment
Table 4
Results of the method validation for tetracyclines, aminoglycosides, quinolones, and lincosamides families.
Analytes
Transition 1 “major”
Transition 2 “minor”
Fc/-value
CCβ
LOD (μg kg−1)
Sens (%)
Fc/-value
CCβ
LOD (μg kg−1)
Sens (%)
Tetracyclines
Chlortetracycline
Fc > T
<
<1
100
Fc > T
<
<1
100
Doxycycline
Fc > T
<
<1
100
Fc > T
<
7.36
100
Oxytetracycline
Fc > T
<
<1
100
Fc > T
<
<1
100
Tetracycline
Fc > T
<
<1
100
Fc > T
<
1.08
100
Aminoglycosides
Amikacin
Fc > T
<
3.71
100
Fc > T
<
3.29
100
Apramycin
Fc > T
<
7.60
100
Fc > T
<
5.22
95
Dihydrostreptomycin
Fc > T
<
<1
100
Fc > T
<
<1
95
Gentamicin
Fc > T
<
4.68
100
Fc > T
<
5.44
100
Hygromycin
Fc > T
<
<1
100
Fc > T
<
<1
100
Kanamycin
Fc > T
<
<1
100
Fc > T
<
4.87
100
Neomycin
Fc > T
<
6.22
100
Fc > T
<
6.91
100
Spectinomycin
Fc > T
<
<1
95
Fc > T
<
<1
100
Streptomycin
Fc > T
<
<1
100
Fc > T
<
<1
100
Tobramycin
Fc > T
<
5.18
100
Fc > T
<
7.01
100
Quinolones
Ciprofloxacin
Fc > T
<
<1
100
Fc > T
<
<1
100
Enrofloxacin
Fc > T
<
<1
100
Fc > T
<
<1
100
Flumequine
Fc <T
>
<1
100
Fc > T
<
<1
100
Lincomycin
Fc > T
<
<1
100
Fc > T
<
<1
100
Nalidixic acid
Fc > T
<
<1
100
Fc > T
<
<1
100
Norfloxacin
Fc > T
<
<1
100
Fc > T
<
<1
100
Oxolinic acid
Fc > T
<
<1
100
Fc > T
<
<1
100
Sarafloxacin
Fc > T
<
<1
100
Fc > T
<
<1
100
Lincosamides
Lincomycin
Fc > T
<
<1
100
Fc > T
<
<1
100
Fc: cut-off factor; LOD: limit of detection; Sens: sensitivity of the method; : level of interest for each analyte according to Table 1.
