Validation of an UHPLC-MS/MS Method for Screening of Antimicrobial Residues in Eggs and Their Application to Analyses of Eggs from Laying Hens Subjected to Pharmacological Treatment

Table 5

Results of the method validation for β-lactams, sulfonamides, and macrolides families.

Analytes	Transition 1 “major”				Transition 2 “minor”
	Fc/-value	CCβ	LOD; (μg kg^−1)	Sens; (%)	Fc /-value	CCβ	LOD; (μg kg^−1)	Sens; (%)
β-lactams
Cefazolin	Fc >	<	<1	100	Fc >	<	2.98	100
Cloxacillin	Fc >	<	<1	100	Fc >	<	<1	100
Dicloxacillin	Fc >	<	<1	100	Fc >	<	2.10	100
Nafcillin	Fc >	<	<1	100	Fc >	<	2.35	100
Oxacillin	Fc >	<	<1	100	Fc >	<	<1	100
Penicillin G	Fc >	<	<1	100	Fc >	<	<1	100
Penicillin V	Fc >	<	<1	100	Fc >	<	1.76	100
Sulfonamides
Sulfachloropyridazine	Fc >	<	<1	100	Fc >	<	<1	100
Sulfadiazine	Fc >	<	<1	100	Fc >	<	<1	100
Sulfadimethoxine	Fc >	<	<1	100	Fc >	<	<1	100
Sulfadoxine	Fc >	<	<1	100	Fc >	<	<1	100
Sulfamerazine	Fc >	<	<1	100	Fc >	<	<1	100
Sulfamethazine	Fc >	<	<1	100	Fc >	<	<1	100
Sulfamethoxazole	Fc >	<	<1	100	Fc >	<	<1	100
Sulfamethoxypyridazine	Fc >	<	<1	100	Fc >	<	<1	100
Sulfaquinoxaline	Fc >	<	<1	100	Fc >	<	<1	100
Sulfathiazole	Fc >	<	<1	100	Fc >	<	<1	100
Sulfisoxazole	Fc >	<	<1	100	Fc >	<	<1	100
Macrolides
Clindamycin	Fc >	<	<1	100	Fc >	<	<1	100
Erythromycin	Fc >	<	<1	100	Fc >	<	<1	100
Spiramycin	Fc >	<	<1	100	Fc >	<	<1	100
Tilmicosin	Fc >	<	<1	100	Fc >	<	<1	100
Tylosin	Fc >	<	<1	100	Fc >	<	<1	100

Fc: cut-off factor; LOD: limit of detection; Sens: sensitivity of the method; : level of interest for each analyte according to Table 1.