Evaluation of Global Genomic DNA Methylation in Human Whole Blood by Capillary Electrophoresis UV Detection

Figure 2

Typical electropherogram of the DNA sample extracted from whole human blood after formic acid hydrolysis obtained with 60 mmol/L sodium acetate buffer at pH 3.60 as the run buffer. Electrophoretical conditions: uncoated silica capillary, 60 cm × 100 μm id; cartridge temperature, 18°C; voltage, 22 kV; detection, 280 nm; hydrodynamic injection, 270 nL (2.0 psi × 5 s). Cyt = cytosine; mCyt = 5-methylcytosine.